BioPorto Diagnostic

AntibodyShop

Anti GLP-1(7-37) and GLP-1(7-36) amide (free N-terminus-specific)

Mouse monoclonal antibody, biotinylated

Cat.No. ABS 033-10B

Subclass lgG2a/k

Presentation:

Preparation: Biotinylated
Content: 50 無, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Immunogen:

KLH-conjugated synthetic [Cys18]-GLP-1(7-17) adsorbed onto aluminum hydroxide gel

Specificity:

ABS 033-10 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide and shows <0.2% cross-reactivity with GLP-1(9-36) amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4.

Epitope specificity:

Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.

Reactivity:

ABS 033-10 binds free GLP-1(7-37) and free GLP-1(7-36) amide in solution.
ABS 033-10 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well.
ABS 033-10 binds free exendin-4 in solution and can function as a capture antibody for exendin-4.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1/40,000
Immunoblotting	Not determined
Immunohistochemistry	Not determined

Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody for GLP-1 (7-37)coated at 1 痢/ml. Users should determine the optimal dilution for their own purposes